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TUNEL Assay

XT Xiaohua Tan
LY Lixia Yu
RY Ruo Yang
QT Qianyu Tao
LX Lijun Xiang
JX Jian Xiao
JZ Jin-San Zhang
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The Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay is a method for detecting DNA fragmentation. TUNEL has been widely used to identify and quantify cell death in tissues within the last two decades. To assess the protective effect of FGF10 against apoptosis, TUNEL staining and immunohistochemistry staining of cleaved Caspase-3 were used to detect the apoptosis in the kidney tissue after reperfusion. The experimental protocol was exactly the same as we described in our previous study. Kidneys were excised and harvested 1 day after I/R injury. After being dehydrated in gradient ethanol, renal tissue was embedded in paraffin and cut into 5 μm sections. For TUNEL assay, slides were handled with the TUNEL Apoptosis Assay KIT following manufacturing process instructions. The images were captured under a Laser confocal microscope (Nikon, Ti-E&A1 plus). The apoptosis index was analyzed based on five randomly selected images from each group.

Copyright and License information:  ©2020 Tan, Yu, Yang, Tao, Xiang, Xiao and Zhang
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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