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Culture media were removed and washed with PBS for three times, and 0.1% neutral red (in PBS) was added. After 3 h, cells were washed twice with PBS, and then the image was got with an optical microscope or the quantification was obtained with lysis solution (1:1 of 0.1 M acetic acid and 100% ethanol) for 6 h, absorbance was measured at 570 nm with a Spectra (Shell) Reader.
Copyright and License information: ©2020 Gong, Tai, Huang, Xiao, Mo, Wang, Han, Zhou, Chen, Tang, Zhao, Xu, Gong, Zhang, Yang, Wang and Xiao.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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