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Tunel Assay

LL Lin Li
KW Ke Wan
LX Linkai Xiong
SL Shuang Liang
FT Fangfang Tou
SG Shanxian Guo
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After dewaxed by xylene and hydrated by gradient ethanol, xenograft tumor tissue sections were treated with Proteinase K for 30 min at room temperature. Then, sections were incubated with 3% H2O2 and 0.1% TritonX-100 for 10 min. According to the Tunel kit instructions (Roche, USA), sections were biotinylated and then stained with DAB and hematoxylin. Before being subjected to gradient ethanol dehydration, sections were washed 3 times with water. Xylene was used to treat sections and neutral resin was used to seal sections. All the sections were observed and photographed under a microscope.

Copyright and License information:  ©2020 Li et al.
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