Caspase 3/7 activity assay

SL Shun Mei Lin
CB Chan Yu Baek
JJ Jong-Hyun Jung
WK Woo Sik Kim
HS Ha-Yeon Song
JL Ji Hee Lee
HJ Hyun Jung Ji
YZ Yong Zhi
BK Bo Sun Kang
YB Yong-Sun Bahn
HS Ho Seong Seo
SL Sangyong Lim
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Caspase 3/7 activity was analyzed using a Caspase-3/7 fluorescence assay kit (Cayman Chemical; Ann Arbor, MI, USA) following the manufacturer’s protocols. In brief, 104 NHEK-Ad cells were plated into 96-well cell culture plates (SPL). At 80% confluence, the cells were irradiated with 120 mJ/cm2 UV light with or without pre-treatment of DeinoPol (10 μg). After 3 h, cells were lysed, followed by incubation of 90 μL cell lysate and 10 μL caspase 3/7 substrate solution. The fluorescence intensity of each well was measured with excitation 485 nm and emission 535 nm using a Victor X3 light plate reader (PerkinElmer).

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