Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Wound healing assay
Ask a
question
Favorite
HepG2 and SMMC-7721 cells were seeded into 6-well plates and grown to 90% confluence. Then, the confluent cell monolayers were scraped with a 10 μL pipette tip to create wounds. The cellular debris was removed and cells were cultured in FBS-free medium. Pictures were taken under a microscope (Leica) at 0, 24, and 48 h. The difference in wound width represents the migration ability.
Copyright and License information: ©2018 The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
