2.6. Antibacterial Activity

In order to study the effect of the samples on the microbial growth, E. coli (ATCC 25922) and P. aeruginosa (ATCC 10145) were used. E. coli was cultured in TBX Medium (Tryptone Bile X-Gluc) (Liofilchem, Roseto degli Abruzzi, Italy), while P. aeruginosa in Pseudomonas CN Agar (Liofilchem, Roseto degli Abruzzi, Italy).

Afterwards, the bacterial cultures were diluted in distilled water to produce a bacterial cell suspension of 10 × 10⁵ CFU/mL. Both bacteria were inoculated in absence and in presence of the hybrid materials. E. coli was incubated with the materials for 24 h at 44 °C, while the P. aeruginosa for 48 h at 36 °C. The microbial growth was evaluated by observing the diameter of the inhibition halo (ID). The obtained values are the mean standard (SD) deviation of measurements carried out on samples analyzed three times.