Histomorphological analysis of tissues

For histomorphological analysis, the healed tissues from the animals of each group were taken at the end of the experiment (after 24 days), fixed in 10% formalin, dehydrated by alcohol and embedded in paraffin blocks. Tissue sections were deparaffinized with the help of xylene. Serial sections of particular diameter were cut with the help of microtome and finally stained using hematoxylin-eosin (HE) and analyzed under light microscopy. In the healed areas, the possibility of the inflammation was estimated in a blind manner by counting the inflammatory cell infiltration/field. The presence of “epithelization, inflammatory cell infiltration, fibroblast proliferation, neovascularization and collagen deposition” on healed area of tissues were determined using a modified 0–5 numerical scale reported in literature (Alam et al. 2018).