Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
In Vitro Tubulin Polymerization Assays
Request a Protocol
Ask a
question
Favorite
Tubulin polymerization assays were conducted using the HTS-Tubulin polymerization assay kit (Cytoskeleton Inc.) as described previously.10 The final concentration of test compounds was 50 μM in 0.5% DMSO. Tubulin polymerization kinetics were measured at 37 °C in a Tecan microplate reader (Tecan Group Ltd.) at 340 nm every minute for 1 h.
Copyright and License information: ©2016
This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
