Cell player YOYO-1 cytotoxicity assay

Samuel Engelsgjerd; Selvi Kunnimalaiyaan; Emad Kandil; T. Clark Gamblin; Muthusamy Kunnimalaiyaan

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Cell player YOYO-1 cytotoxicity assay

SE Samuel Engelsgjerd

SK Selvi Kunnimalaiyaan

EK Emad Kandil

TG T. Clark Gamblin

MK Muthusamy Kunnimalaiyaan

This method is extracted from research article: PLoS One, Mar 2019

Xanthohumol increases death receptor 5 expression and enhances apoptosis with the TNF-related apoptosis-inducing ligand in neuroblastoma cell lines

DOI: 10.1371/journal.pone.0213776

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This assay is based on the membrane integrity of the cells and the use of YOYO-1 iodide (491/509) (Life Technologies), 491/509, a fluorescent dye and CellPlayer Kinetic Caspase3/7 Apoptosis reagent (Life Technologies). Cells were plated in 96-well plates and incubated overnight. Then the cells were treated with various concentrations of xanthohumol, YOYO-1 (1:10000), and CellPlayer (1:10000), and continued to incubate in an Incucyte for 96 hours. Images of both phase-contrast and fluorescence were collected every 2–4 hours and analyzed using IncuCyte 2011A (Essen Bioscience Inc, Ann Arbor, MI) software.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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