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Generation of 3xHA tagged Pkhd1 knock-in mouse lines

JK Jun-ya Kaimori
CL Cheng-Chao Lin
PO Patricia Outeda
MG Miguel A. Garcia-Gonzalez
LM Luis F. Menezes
EH Erum A. Hartung
AL Ao Li
GW Guanqing Wu
HF Hideaki Fujita
YS Yasunori Sato
YN Yasuni Nakanuma
SY Satoko Yamamoto
NI Naotsugu Ichimaru
ST Shiro Takahara
YI Yoshitaka Isaka
TW Terry Watnick
LO Luiz F. Onuchic
LG Lisa M. Guay-Woodford
GG Gregory G. Germino
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The Pkhd1 HA-Flox mouse line will be described elsewhere (manuscript in review). Briefly, this line has exon 67 flanked by lox P sites and three copies of the HA tag “knocked-in” in-frame to the C-terminus of FPC in exon 67. In our previous in vitro studies, we had found that similar modification did not change the trafficking properties of FPC18 in MDCK cells. After establishing a heterozygous Pkhd1 HA-Flox line, we excised the neo cassette by crossing with a Flp-expressing mouse.

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