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Proliferation of T cells was examined by analysis of intracellular expression of the Ki-67 antigen using anti–Ki-67 mAb. Lymphocytes from different organs were isolated and stained for surface markers, followed by intracellular staining of Ki-67 with Foxp3 staining buffer (eBioscience). Samples were acquired by flow cytometer and analyzed by FlowJo.
Copyright and License information: ©2019 by the American Society of Nephrology
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