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After treatment of BMSCs with H2O2, nuclear and cytosolic fractions were isolated using the nuclear and cytoplasmic extraction reagents, these nuclei and cytoplasmic lysates were used for Western blot analysis. Equal amounts of proteins were added to the gel for electrophoresis, after electrophoresis, the protein was transferred to a nitrocellulose membrane. Anti‐Nrf2 antibody was incubated overnight, and the second antibody was incubated for 2 hours. Images were taken with the Gel Imaging System and quantified using ImageJ software.
Copyright and License information: ©2019 The Authors. Published by Wiley Periodicals, Inc.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
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