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Luciferase assay for NF-kB activation

RS Ruey-Shyang Soong
RA Ravi K. Anchoori
BY Benjamin Yang
AY Andrew Yang
ST Ssu-Hsueh Tseng
LH Liangmei He
YT Ya-Chea Tsai
RR Richard B.S. Roden
CH Chien-Fu Hung
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HEK 293 cells transiently transfected with a luciferase reporter construct driven by either an NF-κB-dependent promotor (NF-κB/FL) or a constitutive promoter (Luc) were treated with RA190 or Bortezomib, and TNFα (10 ng/ml) for 7 h. Upon the addition of luciferin, bioluminescence was measured in cell lysates using a luminometer.

Copyright and License information:  ©2016 Soong et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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