Genotyping of Y-chromosome STRs

Seventeen Y-chromosomal STR loci were amplified using the AmpFlSTR Yfiler PCR Amplification kit, following the manufacturer’s instructions (User’s Manual, Applied Biosystems). Separation and detection of the amplified products were performed in an Applied Biosystems PRISM 310 genetic analyzer. Allele assignations were performed by comparison with a reference ladder included in the kit, and using the nomenclature recommended by the International Society for Forensic Genetics (ISFG) [32]. These analyses were performed in the laboratory of the Population Genetics and Identification group, of the Genetics Institute of the Universidad Nacional de Colombia.

The Y- chromosomal data for all 83 donors have been submitted to the open-access Y-STR Haplotype Reference Database (YHRD, www.yhrd.org): accession number YA004510.