Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Spinal nerve ligation neuropathic pain model establishment in rats
Ask a
question
Favorite
To produce a spinal nerve ligation (SNL) model, the rats were anesthetized with isoflurane inhalation in 100% oxygen (induced at 5% and maintained at 2%) and placed in a prone position. A midline incision was made at the L4–S2 level. The left paraspinal muscles were separated from the spinous processes, and the L6 transverse process was removed to expose the L4 and L5 spinal nerves. The L5 spinal nerve was then isolated and tightly ligated with 4-0 silk thread36. A sham surgery involved the same procedure without the ligation of the L5 spinal nerve.
Copyright and License information: The Author(s) ©2019
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
