Intracerebroventricular (i.c.v) injections

WT and IL17 -/- mice were administered BDNF (0.1 mg/mL, total volume of 3 μl), saline solution (PBS, total volume of 3 μl) or anti-mouse IL-17 (0.8 mg/mL, total volume of 5 μl) (Clone 17F3, BioXCell) and IgG1 isotype control (0.8 mg/mL, total volume of 5 μl) (Clone MOPC-21) by i.c.v. injection as previously described (35). Briefly, mice were anesthetized under 1.5% isoflurane in 100% oxygen in a transparent acrylic chamber. After the induction, mice were moved out of the chamber to a stereotaxic frame, and isoflurane anesthesia was maintained. A single i.c.v was performed into the right ventricle of the brain, using the stereotaxic coordinates of 0.6 mm posterior, 1.2 mm lateral and 2.2 mm ventral to Bregma. A 10 μl hamilton syringe was used for i.c.v injection. Behavioral assessment was performed 24h after surgery in the Y-maze.