Cell transfection and virus Infection

YW You Wu
WS Wanyue Shi
TT Tingting Tang
YW Yidong Wang
XY Xin Yin
YC Yanlin Chen
YZ Yanfeng Zhang
YX Yun Xing
YS Yumeng Shen
TX Tiansong Xia
CG Changying Guo
YP Yi Pan
LJ Liang Jin
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For transient transfection, miRNA mimics, inhibitors, siRNAs (Genepharma), and plasmids were transfected using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol. Lentivirus encoding miR-29a (Genepharma) or SUV420H2 were imported into MCF-7 cells as previously described17. The clones with the stable miR-29a or SUV420H2 expression were selected by green fluorescence protein (GFP) expression.

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