For transient transfection, miRNA mimics, inhibitors, siRNAs (Genepharma), and plasmids were transfected using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol. Lentivirus encoding miR-29a (Genepharma) or SUV420H2 were imported into MCF-7 cells as previously described17. The clones with the stable miR-29a or SUV420H2 expression were selected by green fluorescence protein (GFP) expression.
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