Bacteria-induced prostatitis model

The CP1 E. coli bacterial strain was kindly provided by A. Schaeffer and colleagues (Johns Hopkins University) and was initially isolated from the prostatic secretions of a patient with chronic prostatitis (Rudick et al., 2011). Bacterial cultures and transurethral inoculation were performed as previously described (Simons et al., 2015). Mice were inoculated with a single dose of CP1, or PBS, by transurethral infection.