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Transfected MKN-45 and AGS cells (4 × 103 cells/well) were seeded in RPMI-1640 medium (10% FBS) for 24, 48, 72, or 96 h. Next, MKN-45 and AGS cell suspensions were incubated with 20 μL of MTT for 4 h. After removing the MTT solution, 150 μL of dimethyl sulfoxide was added. Finally, cell viability was assessed using a microplate reader to determine the optical density at 490 nm.
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