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2.7 Measurement of serum inflammatory cytokine concentrations

DK Dean P J Kavanagh
AL Adam B Lokman
GN Georgiana Neag
AC Abigail Colley
NK Neena Kalia
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Circulating cytokine concentrations were identified in serum samples from mice following sham surgery, IRI, or IRI+HSPC administration within 5 min of reperfusion. Reperfusion was performed for 1 h. Serum was isolated by centrifugation (2000 rpm, 10 min). Samples were loaded in triplicate onto a Bio-Plex pro mouse cytokine 23-plex assay plate (Bio-Rad Laboratories, CA, USA) and analysed using a Luminex 200 plate reader (Bio-Rad Laboratories, CA, USA) following the manufacturer’s instructions.

Copyright and License information:  ©2019-05-07 The Author(s) 2019. Published by Oxford University Press on behalf of the European Society of Cardiology
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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