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RNA Extraction and qRT-PCR.

MN Maria A. Nohales
SK Steve A. Kay
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As previously described (28), total RNA was isolated with the GeneJET plant RNA purification mini kit (Thermo Scientific). For cDNA synthesis, 1 μg of total RNA was digested with DNase I (Roche) and reverse-transcribed using the iScript cDNA synthesis kit (Bio-Rad). Synthesized cDNA was amplified by real-time quantitative PCR (qPCR) with Maxima SYBR Green qPCR Master Mix (Thermo Scientific) using the CFX-384 Real Time System (Bio-Rad). PROTEIN PHOSPHATASE 2A (PP2A) (AT1G13320) was used as the normalization control. Primer sequences are listed in Table S1.

Copyright and License information:  ©2019 the Author(s). Published by PNAS.
This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).

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