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Cell cycle analysis
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Cells (1 × 106 cells per well) were seeded in 6-well plates and incubated for 24 h. Cells were collected and fixed in 1 ml of 70% ice-cold ethanol, incubated at 4 °C for 2 h, and centrifuged at 1000×g for 5 min to remove the ethanol. Cellular pellets were washed with PBS and suspended in 0.5 ml of PBS containing 50 µg/ml RNase A for 30 min at 37 °C. Then propidium iodide (50 µg/ml) staining solution was added, and cells were incubated for 30 min at 37 °C in the dark. The samples were measured by flow cytometry to determine the cell cycle distribution.
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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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