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2.6. Markers of ROS Production

MV Matthew C. Valdez
SS Samantha J. Snow
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NADH-ubiquinone reductase (UBIQ-RD) was selected as a marker of ROS production as it plays a critical role in several neurodegenerative diseases in which OS is a potential responsible pathway. UBIQ-RD activity was assayed following the method of Cormier et al. [33] where the enzyme catalyzes the oxidation of NADH+ H+ to NAD+, with the ultimate reduction of ubiquinone to ubiquinol. The rate of UBIQ-RD activity was measured as a rotenone-sensitive rate of NADH oxidation at 37 °C and 340 nm.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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