4.2. Construction of Recombinant Binary Vector

Since none of the tested Avr genes contained predicted introns, we amplified each of the genes directly from genomic DNA from the P. infestans HLJ, using the Cobuddy DNA Polymerase (CWBIO, Beijing, China) and specific primers. The PCR products were digested with the appropriate enzymes and cloned into the recombinant binary vector pEarleyGate 102 (C-CFP-HA) [59] and PVX vector pGR106. The constructs were validated by DNA sequencing then introduced into Agrobacterium tumefaciens GV3101 or AGL1.