Female BALB/c or C57BL/6 mice of six to eight weeks of age were obtained from Charles River (Wilmington, MA) for young mouse vaccination studies as well as in vitro APC studies. Aged female BALB/c mice of ≥ 20 months of age were obtained from Jackson Laboratory (Bar Harbor, ME). All studies involving the use of animals was carried out in accordance with current institutional guidelines for the care and use of animals.
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