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The good epochs were averaged and the amplitude of the N30 peak was calculated from channel F3 [15], contralateral to the stimulated nerve. The most positive and the most negative peaks were identified automatically in the windows from 15–25 ms and 25–35 ms, respectively. The identified peaks were manually verified and modified by an expert if (i) they were out of this time period or (ii) there was more than one peak in a window, which lead to the wrong identification of the peak. The N30 amplitude was taken as the absolute difference of the amplitudes of these two peaks.

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