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Flow cytometry analysis of apoptotic cells
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Cells (2 × 105 cells/well) were seeded into 6-well plates and cultured for 24 hr. Gefitinib and/or PD 0332991 were added at various concentrations and cells were cultured for another 24 hr. Cells were stained using an Annexin V-FITC Apoptosis Analysis Kit (BD Bioscience, CA, USA) and analyzed with a FACSAria™ flow cytometer (BD Bioscience, CA, USA).
Copyright and License information: ©2016 Liu et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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