2.2. Eligibility criteria

Peer-reviewed journal articles that meet eligibility criteria based on study population, interventions, control, and outcomes (PICOS)^[16] will be included in the review.

The review will include original articles resulting from experimental studies carried out in rats or mice of both sexes, without water or diet restriction, with diagnosis of intestine inflammation at the beginning of the experiment; studies evaluating therapy with anti-TNF-alpha molecule and the effect of this treatment on the intestinal barrier (protection/recovery/damage or lack of effect); studies with intestinal barrier cells of rats or mice that evaluated the same treatment and measured the same outcomes previously cited.

Review articles, case reports, comments, editorials, letters to the editor, theses, conference proceedings will be excluded; studies with other animal/cell models; studies evaluating anti-TNF-alpha treatment in other inflammatory diseases; studies that do not present at least one measure of TNF-alpha reduction or its activity; studies that do not address mechanisms of action of the molecules studied to obtain the effects found.