2.1. Materials

AmB was procured from North China Pharmaceutical Huasheng Co. Ltd. (Hebei, China). Hydrogenated soy phosphatidylcholine (HSPC), distearoylphosphatidylglycerol (DSPG), cholesterol, and hydrochloric acid (37%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hanmi Gas Co. Ltd. supplied carbon dioxide with a high purity of 99.9% (Seoul, Republic of Korea). Ethyl alcohol (purity 99.9%), methyl alcohol (MeOH, purity 99.5%), chloroform (CHCl₃, purity 99.5%), dimethyl sulfoxide (DMSO, purity 99.5%), N,N-dimethylacetamide (DMA, purity 99.5%), and N,N-dimethylformamide (DMF, purity 99.5%) were purchased from Samchun Pure Chemical (Pyeongtaek-si, Republic of Korea). All other chemicals and reagents were of analytical grade. Purified water of Milli-Q quality (Milli-Q Reference, Millipore^®, Molshiem, France) was used throughout the study.