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Measurement of Triglycerides and Insulin.
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For TG measurement, brown adipose tissues were homogenized in lysis buffer (140 mM NaCl, 50 mM Tris pH 7.4, and 1% Triton X-100). TG concentration was then measured in both BAT and blood serum using the LiquiColor Triglyceride kit following the manufacturer’s protocol (StanBio). Insulin was measured in blood serum using mouse ultrasensitive ELISA kit (Crystal Chem).
Copyright and License information: ©2019
Published under the PNAS license.
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