4.1. Reagents

All cell culture plates and general-purpose reagents were obtained from Corning Cellgro (Manassas, VA, USA). Human recombinant VEGF-₁₆₅ was obtained from the NCI Bulk Cytokine and Monoclonal Antibody Preclinical Repository (Frederick, MA, USA). ¹²⁵I-VEGF₁₆₅ was prepared using Bolton–Hunter reagent obtained from Perkin Elmer (Boston, MA, USA). Human plasma fibronectin was obtained from Millipore (Temecula, CA, USA). Vybrant Cell Labeling Solutions were purchased from In Vitrogen Molecular Probes (Eugene, OR, USA). Monoclonal antibodies to heparan sulfate (clones 3G10 and 10E4) were obtained from Northstar BioProducts (East Falmouth, MA, USA) and the HRP linked anti-IgG secondary antibodies were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). Heparinase III (E.C. 4.2.2.8) was from Biomarin Pharmaceuticals (Montreal, Canada). Recombinant Fc-VEGF Receptor 1 (#321-FL) and Fc-VEGF Receptor 2 (#357-KD) chimera proteins containing the extracellular domain of the human receptors were purchased from R & D Systems (Minneapolis, MN, USA). DNAse I was obtained from New England Biolabs (Beverly, MA, USA) and the RNAse I inhibitor was obtained from Life Technologies (Grand Island, NY, USA). All other chemicals were reagent grade products obtained from commercial sources.