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Cells (1.2 × 105 cells/well) were seeded in a 6-well plate for 24 h prior to incubation with diverse concentrations of analogs for 5 h. To measure intracellular ATP levels, we used a luminometric-based ATP Assay kit according to the manufacturer’s protocol. The signal was measured by a Luminoskan luminometer (Thermo Scientific, Atlanta, GA, USA). The ATP concentration with each treatment was calculated as a percentage of that of the control.
Copyright and License information: The Author(s). ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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