4.7. Cell Proliferation Assay

The effect of TGF-β on cell proliferation in different cell lines was measured by CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, WI, USA). Cells were grown in 2D (96 well plate BD) and 3D anchorage-independent condition using ultra-low attachment 96 well plate (Corning, Sigma-Aldrich, New York, NY, USA) with seeding density of 5000 cells per well for 3 days. The effect of cell proliferation in the presence of TGF-β and {"type":"entrez-nucleotide","attrs":{"text":"LY364947","term_id":"1257906561","term_text":"LY364947"}}LY364947 (Tocris Bioscience, Minneapolis, MN, USA) in 2D and 3D models was measured.