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Blood was drawn from healthy, nonatopic donors in syringes containing 0.1 M citrate, followed by Ficoll gradient (GE Healthcare) centrifugation. Erythrocytes were lysed, and all of the following steps were performed at 4°C. Eosinophil isolation was performed by negative immunomagnetic selection, as previously described.31 Cells were pelleted by centrifugation for 10 minutes at 300g. Purity of isolation was verified by FACS for Siglec-8 and was confirmed to be >98%; there was no contamination with platelets as assessed by FACS for CD41.
Copyright and License information: ©2019 by The American Society of Hematology
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