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Rat hearts were divided into atria and ventricles, and isolated left atria (LA) were fixed in 4% paraformaldehyde for 24 hours, embedded in paraffin, and sectioned transversely at 5 μm. Masson trichrome staining was used to evaluate interstitial fibrosis. Images were visualized by light microscopy (ECLIPSE 80i; Nikon, Japan). The LA fibrotic area was analyzed at ×400 magnification as the percentage of area of positive fibrotic staining divided by total myocardial tissue areas by using image analysis system software (Image‐Pro Plus version 6.0).
Copyright and License information: ©2019 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
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