2.7. Determination of Cellular Migration by Wound Healing Assay

To determine the migration ability of ECs by wound healing assay, wild-type and SLPI-overexpressing EA.hy926 monolayer scratch cells were seeded into a 6-well plate and cultured to >90% confluence [15]. Then, scratch wounding was performed using a 200 µL pipette tip. After that, cell debris and loose cells were removed by washing twice with PBS before changing to fresh completed medium. Migration was monitored for 24 h by taking photographs. Quantification of the area was calculated by ImageJ software via the following equation: [1 − (A_t=24/A_t=0)] × 100%, where A is the area achieved and t is the time point at 0 or 24 h.