Nude mouse xenograft assays

ZZ Zhifa Zhang
ZP Zhaohui Peng
JC Junying Cao
JW Jiaqi Wang
YH Yongyu Hao
KS Kai Song
YW Yan Wang
WH Wei Hu
XZ Xuesong Zhang
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3 × 106 indicted NSCLC cells were subcutaneously injected into 6-week-old athymic BALB/c nude mice purchased from the Shanghai Experimental Animal Center of Chinese Academy of Sciences (Shanghai, China). Subcutaneous xenograft growth was measured weekly with a caliper, and the tumor volume was calculated as a × b2 × 0.5 (a, longest diameter; b, shortest diameter). The animal studies were approved by the ethics committee of the General Hospital of Chinese People’s Liberation Army (Beijing, China).

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