Passive avoidance test

Passive avoidance test was performed for measuring memory retention in mice according to a previous study [38]. Briefly, the mice were placed individually in the illuminated compartment of automated passive avoidance system. By pressing the pedal, the apparatus was activated. The guillotine door was raised after 10 seconds allowing the mice to enter the dark compartment. The door was then closed and mice were given a foot-shock (0.2mA), for 2 seconds on training day. 24h after the training session, memory errors and step-through latency were recorded. If mice did not enter the dark compartment within 5min, the mice were assigned a score of 300 seconds.