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Cell culture supernatants from different experimental conditions were examined for extracellular IL-1β concentration using Thermo Fischer Scientific ELISA kits (Waltham, MA, USA, detection limit 4 pg/mL). Intracellular IL-1β was determined in lysates obtained after three freeze–thaw cycles and resuspended in PBS [21].
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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