Intraocular Injections in mice

For intravitreal injection, recombinant proteins or cells in 1μl volume were injected into the right eye using a graduated pulled glass pipet and a wire plunger (Wiretrol II, 5-0000-2005, Durmmond Scientific Company). For details on the test articles injected and procedure for intravitreal injections see Supplementary Materials.

For sub-retinal injection, dissociated GFP-expressing mouse retinal cells from the Nrl-GFP mice were transplanted into the subretinal space of recipient mice using the trans-corneal subretinal injection method. For details on the preparation Nrl-GFP cells and procedure for transplantation see Supplementary Materials.