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One thousand PCCs or PSCs per well were plated in triplicate into 96‐well plates with DMEM containing 10% FBS for 24 h. After cellular adhesion to the plates, the medium was replaced with fresh DMEM containing 10% FBS plus calpeptin at 0 (DMSO), 10, 20, 40, 60, or 80 μM (day 0). Cell viability was determined with a CellTiter‐Glo luminescent cell viability assay kit (Promega, Madison, WI, USA) according to the manufacturer's instructions on days 0–4 every 24 h.
Copyright and License information: ©2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.
This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
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