Immunoblot analysis

Cells or phagosomes were lysed directly in 2× Laemmli buffer, separated on 4‐12% Nu‐PAGE gels (Invitrogen) and immunoblotted onto PVDF membranes (Amersham). Membranes were blocked for 1 h in 5% (w/v) skim milk in TBS containing 0.1% (v/v) Tween 20 and subsequently incubated with different primary antibodies overnight. After incubation with HRP‐labelled secondary antibodies, proteins were detected using ECL and X‐ray films. Immunoblots were quantified in ImageJ software. For activation of MSR1, WT and MSR1 KO BMDMs were either stimulated with fucoidan (50 μg/ml, Sigma‐Aldrich) for 1 h alone or with IL‐4 (20 ng/ml, RD) for 24 h followed by fucoidan 50 μg/ml, Sigma‐Aldrich) treatment for 1 h.