4.3. RNA Extraction

Samples (10 mosquitoes pool for each sample and three replicates for each time point) were homogenized with a plastic pestle in a 1.5 mL tubes. Total RNAs were extracted using the TRIzol reagent according to the manufacturer’s instruction (Ambion, Life Technologies, Carlsbad, CA, USA) and followed the standard protocol [35,39]. The RNA samples were digested by DNase I (RNase-free), according to the manufacturer’s instructions (Thermo Scientific, Wilmington, DE, USA). The purified RNA samples were quantitated by NANODROP 2000 Spectrophotometer (Thermo Scientific, Wilmington, DE, USA).