5.3. ROS measurements with chemiluminescence

ROS production was assessed in intact cells with 100 µmol/L luminol (from SigmaAldrich) / 1 U/ml horseradish peroxidase (HRP, from SigmaAldrich) for H₂O₂ or with 200 µmol/L L-012 (from Wako Chemicals) for •O₂ˉ in a Berthold TriStar2 microplate reader (LB942, Berthold, Wildbad, Germany). All measurements were performed in HEPES-Tyrode buffer containing in mmol/L: 137 NaCl, 2.7 KCl, 0.5 MgCl₂, 1.8 CaCl₂, 5 glucose, 0.36 NaH₂PO₄, 10 HEPES.