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One thousand cells were seeded into 96-well plates to adhere overnight. Cells were then incubated with indicated treatments for 24h, followed by the addition of 5mg/ml MTT (Sigma, St. Louis, MO). After incubation at 37°C for 4h, the supernatants were removed. 200μl of DMSO were added, and the absorbance value (OD) at 570nm was measured using an ELISA reader system (TECAN-infinite M200 pro, Mannedorf, Switzerland). The combination index (CI) was obtained using commercially available software (Calcusyn; Biosoft, Ferguson, MO).
Copyright and License information: ©2016 Piao et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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