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Microarray‐Based Gene Expression Data Analysis

XL Xidong Li
SZ Shouwen Zhang
MW Mingguang Wa
ZL Zhonghua Liu
SH Shunpeng Hu
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The microarray data related to MI were retrieved from the Gene Expression Omnibus (GEO) database (https://www.ncbi.nlm.nih.gov/geo/). Standardized pretreatment of the gene expression data was conducted using the affy package in the R language programming package.22 Based on the microarray data GSE46395, differentially expressed genes (DEGs) were screened using the limma package with |log2FC| >2.0 and adj.P.Val (P value after correction) <0.05 as the threshold.23 The heatmap of the DEGs were constructed. MI‐related genes and protein‐protein interaction were searched in the DisGeNET (http://www.disgenet.org/web/DisGeNET/menu/search?4) and String databases (https://string-db.org/).24, 25 Cytoscape 3.6.0 software was applied to obtain information about gene interaction and construct gene interaction network.26 The putative miRs regulating DEGs were predicted in the TargetScan (http://www.targetscan.org/vert_71/).

Copyright and License information:  ©2019 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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