Western blotting

For western blotting analysis, cellular proteins were prepared from pancreas by standard methods. Protein concentration were determined (Bio-Rad Protein Assay) and adjusted to 4 μg/μL. The samples were boiled at 95 °C for 5 min before loading, and then subjected to SDS-polyacrylamide gel electrophoresis and blotted to PVDF membranes. The membranes were probed with the following antibodies: anti- COL1A1 (Santa Cruz Biotechnology Inc. sc-25974). A low-molecular weight protein marker was used to determine the size of detected bands in the western blot.