IPA

A core analysis was performed through the use of IPA (QIAGEN Inc.; https://www.qiagenbioinformatics.com/products/ingenuity-pathway-analysis/) on genes significantly altered between healthy skin Tregs and healthy skin Teffs (adjusted P value < 0.05, log₂ fold change ±1) and on genes significantly altered between peripheral blood Tregs and peripheral blood Teffs (adjusted P value < 0.05, log₂ fold change ±1). Upstream regulators that were identified as shared were significantly (P < 0.05 for upstream regulator analysis) increased or decreased in both skin and blood (Z score ±2). Divergent regulators are depicted, which are significantly increased or decreased (P < 0.05 for upstream regulator analysis) in skin and blood, with a Z score ±2 in skin and a Z score in the opposite direction in blood. Some regulators were assigned biological activity (i.e., siroliumus was assigned as mTORC1 inhibition), and original regulator nomenclature is shown in Supplemental Figure 11.