Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Inhibition of NE activity by sivelestat was confirmed by incubating 1 μM NE and 10 μM Suc-Ala-Ala-Ala-MCA substrate (MAA-3133-v; Peptides International) with or without 1 μM sivelestat for 90 min at 37°C. Substrate fluorescence was measured every 5 min as an indicator of NE activity at excitation/emission wavelengths of 380/460 nm, using a Tecan Infinite M1000 multiplate reader.
Copyright and License information: ©2016, American Society for Microbiology. All Rights Reserved.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
