Mitochondrial swelling assay

Brain mitochondria were prepared as previously described [43]. The appropriate amount of mitochondria was determined for mitochondrial swelling in 1 mL of mitochondrial isolation buffer. Mitochondrial swelling was triggered by the addition of calcium (200 μM). Swelling was observed by immediately and continuously recording changes at OD₅₄₀ nm by using a spectrophotometer at 10 sec intervals for a total of 600 sec.